Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Roseibium aggregatum |
Protein Variants | Comment | Organism |
---|---|---|
D153 N/H191 N | 25fold improved affinity for NADH and with 50fold enhanced catalytic efficiency | Roseibium aggregatum |
D153N | affinity for NADH is improved, 11.3fold decrease in Km value for NADH | Roseibium aggregatum |
H191N | affinity for NADH is improved, 6.0fold decrease in Km value for NADH | Roseibium aggregatum |
P130V | affinity for NADH is improved, 8.6fold decrease in Km value for NADH | Roseibium aggregatum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
NADH | mutant D153N/H191N, pH 8, 30°C | Roseibium aggregatum | |
0.04 | - |
NADH | mutant D153N, pH 8, 30°C | Roseibium aggregatum | |
0.05 | - |
NADH | mutant P130V, pH 8, 30°C | Roseibium aggregatum | |
0.07 | - |
NADH | mutant H191N, pH 8, 30°C | Roseibium aggregatum | |
0.078 | - |
NAD+ | wild-type, pH 8, 30°C | Roseibium aggregatum | |
0.09 | - |
trimethylpyruvate | mutant H191N, pH 8, 30°C | Roseibium aggregatum | |
0.096 | - |
trimethylpyruvate | wild-type, pH 8, 30°C | Roseibium aggregatum | |
0.1 | - |
trimethylpyruvate | mutant D153N/H191N, pH 8, 30°C | Roseibium aggregatum | |
0.12 | - |
trimethylpyruvate | mutant D153N, pH 8, 30°C | Roseibium aggregatum | |
0.3 | - |
trimethylpyruvate | mutant P130V, pH 8, 30°C | Roseibium aggregatum | |
0.43 | - |
NADH | wild-type, pH 8, 30°C | Roseibium aggregatum | |
5.05 | - |
L-tert-leucine | wild-type, pH 8, 30°C | Roseibium aggregatum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Roseibium aggregatum | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
556.6 | - |
substrate trimethylpyruvate, wild-type, pH 8, 30°C | Roseibium aggregatum |
1410 | - |
substrate oxo(phenyl)acetate, wild-type, pH 8, 30°C | Roseibium aggregatum |
1710 | - |
substrate oxo(phenyl)acetate, mutant D153N/H191N, pH 8, 30°C | Roseibium aggregatum |
1743 | - |
substrate 2-oxopropanoate, wild-type, pH 8, 30°C | Roseibium aggregatum |
2217 | - |
substrate 2-oxopropanoate, mutant D153N/H191N, pH 8, 30°C | Roseibium aggregatum |
9046 | - |
substrate 4-methyl-2-oxopentanoate, wild-type, pH 8, 30°C | Roseibium aggregatum |
13099 | - |
substrate 2-aminopentanoate, wild-type, pH 8, 30°C | Roseibium aggregatum |
15871 | - |
substrate 2-oxobutanoate, wild-type, pH 8, 30°C | Roseibium aggregatum |
19609 | - |
substrate 4-methyl-2-oxopentanoate, mutant D153N/H191N, pH 8, 30°C | Roseibium aggregatum |
23654 | - |
substrate 2-aminopentanoate, mutant D153N/H191N, pH 8, 30°C | Roseibium aggregatum |
31256 | - |
substrate 2-oxobutanoate, mutant D153N/H191N, pH 8, 30°C | Roseibium aggregatum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutanoate + NH3 + NADH + H+ | - |
Roseibium aggregatum | L-2-aminobutanoate + H2O + NAD+ | - |
? | |
2-oxopentanoate + NH3 + NADH + H+ | - |
Roseibium aggregatum | L-2-aminopentanoate + H2O + NAD+ | - |
? | |
2-oxopropanoate + NH3 + NADH + H+ | - |
Roseibium aggregatum | L-alanine + H2O + NAD+ | - |
? | |
4-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
Roseibium aggregatum | L-4-methyl-2-aminopentanoate + H2O + NAD+ | - |
? | |
L-tert-leucine + H2O + NAD+ | - |
Roseibium aggregatum | trimethylpyruvate + NH3 + NADH + H+ | - |
? | |
oxo(phenyl)acetate + NH3 + NADH + H+ | - |
Roseibium aggregatum | amino(phenyl)acetate + H2O + NAD+ | - |
? | |
trimethylpyruvate + NH3 + NADH + H+ | - |
Roseibium aggregatum | L-tert-leucine + H2O + NAD+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Roseibium aggregatum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
half-life 72 h | Roseibium aggregatum |
35 | - |
half-life 48 h | Roseibium aggregatum |
40 | - |
half-life 20 h | Roseibium aggregatum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
33.9 | - |
L-tert-leucine | wild-type, pH 8, 30°C | Roseibium aggregatum | |
347 | - |
NADH | wild-type, pH 8, 30°C | Roseibium aggregatum | |
347 | - |
trimethylpyruvate | wild-type, pH 8, 30°C | Roseibium aggregatum | |
571 | - |
NADH | mutant D153N, pH 8, 30°C | Roseibium aggregatum | |
571 | - |
NADH | mutant H191N, pH 8, 30°C | Roseibium aggregatum | |
617 | - |
NADH | mutant P130V, pH 8, 30°C | Roseibium aggregatum | |
688 | - |
NADH | mutant D153N/H191N, pH 8, 30°C | Roseibium aggregatum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Roseibium aggregatum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | more than 70% of maximum activity | Roseibium aggregatum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.7 | - |
L-tert-leucine | wild-type, pH 8, 30°C | Roseibium aggregatum | |
43 | - |
NADH | wild-type, pH 8, 30°C | Roseibium aggregatum | |
434.6 | - |
NAD+ | wild-type, pH 8, 30°C | Roseibium aggregatum | |
808 | - |
NADH | wild-type, pH 8, 30°C | Roseibium aggregatum | |
2058 | - |
trimethylpyruvate | mutant D153N, pH 8, 30°C | Roseibium aggregatum | |
3618 | - |
trimethylpyruvate | mutant P130V, pH 8, 30°C | Roseibium aggregatum | |
3620 | - |
trimethylpyruvate | wild-type, pH 8, 30°C | Roseibium aggregatum | |
4794 | - |
trimethylpyruvate | mutant H191N, pH 8, 30°C | Roseibium aggregatum | |
7165 | - |
trimethylpyruvate | mutant D153N/H191N, pH 8, 30°C | Roseibium aggregatum | |
7936 | - |
NADH | mutant H191N, pH 8, 30°C | Roseibium aggregatum | |
12348 | - |
NADH | mutant P130V, pH 8, 30°C | Roseibium aggregatum | |
15013 | - |
NADH | mutant D153N, pH 8, 30°C | Roseibium aggregatum | |
40465 | - |
NADH | mutant D153N/H191N, pH 8, 30°C | Roseibium aggregatum |